rophoblast stem TS cells using embryonic stem ES cells as a

Last updated: 10/21/2023

rophoblast stem TS cells using embryonic stem ES cells as a control B Nanog Fold enrichment ES TS 2 0 1 5 1 0 0 5 Distal T DMR Input ACH3 AcH4 IgG ES TS ES TS AcH3 AcH4 Fold enrichment Proximal T DMR ES TS 0 5 Input AcH3 AcH4 ES TS AcH3 IgG ES TS AcH4 Oct 4 000 000 000 00 ES TS Fold enrichment 1 0 0 5 T DMR Input AcH3 AcH4 IgG ES TS AcH3 ES TS AcH4 Figure B The ES and TS cells were subjected to ChIP assay using antibodies against acetylated histone H3 AcH3 and H4 AcH4 for the endogenous Nanog gene left sic and the endogenous Oct 4 gene right side The top panels are diagram of the tentat DNA modified regulatory regions T DMRs for both genes Arrows in the top panel indicate the positions of ChIP assay primers Intensity of the bands for AcH3 and AcH was normalized by input indicated as fold enrichment and shown by a histogram in bottom panel Data represent the mean S E in triplicate P 0 01 P 0 05 Student s t test P 0 05 is considered statistically significant White and black bars indicate ES and TS cells respectively Normal rabbit IgG IgG was used as a negative control As a positive control aliquots 0 56 of chromatin fragments were also subjected to PCR without IP input RT reverse transcriptase indicates no RT add