The role of isopropanol or ethanol in plasmid DNA

Last updated: 7/31/2022

The role of isopropanol or ethanol in plasmid DNA precipitation is: 1. Isopropanol/ethanol makes plasmid DNA more soluble in aqueous solution 2. Isopropanol/ethanol makes genomic DNA more soluble while it makes the plasmid DNA less soluble, so plasmid DNA is precipiated 3. Isopropanol/ethanol makes plasmid DNA less hydrophilic (less soluble) in the presence of high salt concentration, so plasmid DNA falls out of the solution 4. Isopropanol/ethanol links DNA to proteins so that the DNA-protein complex precipitates