Biotechnology: Principles and Processes Questions and Answers

Read the instructions on Writing a methods section Using these gu methods section for the experiment you performed today 2 points
Biology
Biotechnology: Principles and Processes
Read the instructions on Writing a methods section Using these gu methods section for the experiment you performed today 2 points
What is the m u map units distance between Mu and Phi 4 3 3 8 17 0 0 0 3 41 8
Biology
Biotechnology: Principles and Processes
What is the m u map units distance between Mu and Phi 4 3 3 8 17 0 0 0 3 41 8
3 8 17 0 0 3 41 8 Question 23 You are a plant breeder interested in using heirloom varieties of tomatoes In particular you are now focused on a region of one of the plant s chromosomes where two genes of interest are located One of them Cr affect the ability of the plant to grow upright and self supported as opposed to procumbent creeping trailing spreading over the ground surface The other gene is responsible for miniature size fruits cherry Ch vs large heavy fruits Procumbent phenotype Cr is dominant over upright Cr and large fruit Cht is dominant over miniature Ch You get the following numbers of offspring from your dihybrid testcross Crt Cht 105 Cr Ch 110 Crt Ch 43 Cr Cht 42 How would you calculate the distance between these genes in cM O 85 300 x 100 O215 300 x 100 O 85 1000 x 100 4 5 pts O42 300 x 100 O 85 215 x 100
Biology
Biotechnology: Principles and Processes
3 8 17 0 0 3 41 8 Question 23 You are a plant breeder interested in using heirloom varieties of tomatoes In particular you are now focused on a region of one of the plant s chromosomes where two genes of interest are located One of them Cr affect the ability of the plant to grow upright and self supported as opposed to procumbent creeping trailing spreading over the ground surface The other gene is responsible for miniature size fruits cherry Ch vs large heavy fruits Procumbent phenotype Cr is dominant over upright Cr and large fruit Cht is dominant over miniature Ch You get the following numbers of offspring from your dihybrid testcross Crt Cht 105 Cr Ch 110 Crt Ch 43 Cr Cht 42 How would you calculate the distance between these genes in cM O 85 300 x 100 O215 300 x 100 O 85 1000 x 100 4 5 pts O42 300 x 100 O 85 215 x 100
2 About ten o clock as I was walking home three guys jumped me I couldn t tell who they were They all wore Frankenstein masks And they shoved me to the ground nd kicked me a few times page 79 a cared about someone something b tricked deceived confused or puzzled someone c attacked suddenly unexpectedly
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Biotechnology: Principles and Processes
2 About ten o clock as I was walking home three guys jumped me I couldn t tell who they were They all wore Frankenstein masks And they shoved me to the ground nd kicked me a few times page 79 a cared about someone something b tricked deceived confused or puzzled someone c attacked suddenly unexpectedly
repeated identical segments also called annuli similar in annelids and arthropods separated by septal
Biology
Biotechnology: Principles and Processes
repeated identical segments also called annuli similar in annelids and arthropods separated by septal
Why are enzymes required for cellular metabolic pathways Select 3 correct answer s The enzymes that are present in a cell determine which metabolic reactions can occur in the cell A single enzyme can convert one substrate into many different products An enzyme lowers the activation energy and therefore increases the rate of a reaction A cell can regulate metabolic pathways by controlling the enzyme concentration in the cell Enzymes are consumed by metabolic pathways when reactants are converted to products
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Biotechnology: Principles and Processes
Why are enzymes required for cellular metabolic pathways Select 3 correct answer s The enzymes that are present in a cell determine which metabolic reactions can occur in the cell A single enzyme can convert one substrate into many different products An enzyme lowers the activation energy and therefore increases the rate of a reaction A cell can regulate metabolic pathways by controlling the enzyme concentration in the cell Enzymes are consumed by metabolic pathways when reactants are converted to products
estion 4 Match the microscope type to the correct description Transmission electron microscope Scanning electron microscope Confocal microscope Fluorescencce microscope Differential interference contrast microscope Phase contrast microscope Dark field microscope Bright field microscope Save Answer A Components of the microscope bring light waves out of phase and produce differences in contrast and brightness B A beam of electrons is spread across the surface of a specimen and topography can be seen C A beam of electrons is sent through a specimen D Light is transmitted through a specimen providing limited contrast Sometimes staining a specimen will enhance contrast but can only be done on fixed specimen E Polarized light is sent via two beams that have different paths through the sample F Light from a laser is focused on a point and scanned across a fluorescently stained specimen in two directions G Light is directed at an angle toward the specimen and a special condenser lens transmits only the light reflected off the specimen H Fluorescent stains absorb light at one wavelength then emit another Question 4 of 4
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Biotechnology: Principles and Processes
estion 4 Match the microscope type to the correct description Transmission electron microscope Scanning electron microscope Confocal microscope Fluorescencce microscope Differential interference contrast microscope Phase contrast microscope Dark field microscope Bright field microscope Save Answer A Components of the microscope bring light waves out of phase and produce differences in contrast and brightness B A beam of electrons is spread across the surface of a specimen and topography can be seen C A beam of electrons is sent through a specimen D Light is transmitted through a specimen providing limited contrast Sometimes staining a specimen will enhance contrast but can only be done on fixed specimen E Polarized light is sent via two beams that have different paths through the sample F Light from a laser is focused on a point and scanned across a fluorescently stained specimen in two directions G Light is directed at an angle toward the specimen and a special condenser lens transmits only the light reflected off the specimen H Fluorescent stains absorb light at one wavelength then emit another Question 4 of 4
monetary gain is justified and further promoted Private individuals and firms not the government own most of the property resources Market institutions have completely addressed production and distribution problems The important consequence of the right of private property is that it encourages investment innovation exchange maintenance of property and economic growth An economy is largely dis embedded from political or social intuitions Question 7 3 points Listen Why does the reported estimated unemployment rate underestimate the unemployment issue in our economy O unemployment rate differentiates between part time and full time workers labor force participation rate is a better indicator for labor market unemployment rate does not count cyclically unemployed workers count discouraged worker as unemployed
Biology
Biotechnology: Principles and Processes
monetary gain is justified and further promoted Private individuals and firms not the government own most of the property resources Market institutions have completely addressed production and distribution problems The important consequence of the right of private property is that it encourages investment innovation exchange maintenance of property and economic growth An economy is largely dis embedded from political or social intuitions Question 7 3 points Listen Why does the reported estimated unemployment rate underestimate the unemployment issue in our economy O unemployment rate differentiates between part time and full time workers labor force participation rate is a better indicator for labor market unemployment rate does not count cyclically unemployed workers count discouraged worker as unemployed
You want to recreate the experiment by Louis Pasteur so you take an environmental sample boil it in water to kill microbes but you find microbial growth occurred anyway Why 2 pts A The presence of endospores abiewola merio eoa B The presence of fruiting bodies of bacteria C The presence of anaerobic bacteria The presence of some microbes by spontaneous generation due
Biology
Biotechnology: Principles and Processes
You want to recreate the experiment by Louis Pasteur so you take an environmental sample boil it in water to kill microbes but you find microbial growth occurred anyway Why 2 pts A The presence of endospores abiewola merio eoa B The presence of fruiting bodies of bacteria C The presence of anaerobic bacteria The presence of some microbes by spontaneous generation due
How many microliters of the PCR buffer do you need to add to the PCR reaction No need to add the microliters unit to your answer just the number PCR Components H O PCR Buffer MgCl2 dNTPs Forward primer Reverse primer DNA template Tag DNA pol Total volume Concentration of the stock sample 20X 75 mM 20 mM 5 M 5 M 0 01 ng L 1 U L Target concentration in reaction tube 1X 1 5 mM 0 2 mM 0 2 M 0 2 M 0 1 ng 50 L 5 U 50 L Volume per reaction 50 L
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Biotechnology: Principles and Processes
How many microliters of the PCR buffer do you need to add to the PCR reaction No need to add the microliters unit to your answer just the number PCR Components H O PCR Buffer MgCl2 dNTPs Forward primer Reverse primer DNA template Tag DNA pol Total volume Concentration of the stock sample 20X 75 mM 20 mM 5 M 5 M 0 01 ng L 1 U L Target concentration in reaction tube 1X 1 5 mM 0 2 mM 0 2 M 0 2 M 0 1 ng 50 L 5 U 50 L Volume per reaction 50 L
1 What is the purpose of using Ammonium Sulfate Precipitation in protein isolation and how does it work 2 Why is it necessary to perform further purification steps after ammonium sulfate precipitation in protein isolation 3 How does ion exchange chromatography contribute to the purification of proteins from spinach leaves and what property of proteins does it exploit 4 Explain the role of NaCl in ion exchange chromatography and how it helps to elute proteins from the column 5 What analytical technique is used to determine protein concentration during ion exchange chromatography and why is this important in the process
Biology
Biotechnology: Principles and Processes
1 What is the purpose of using Ammonium Sulfate Precipitation in protein isolation and how does it work 2 Why is it necessary to perform further purification steps after ammonium sulfate precipitation in protein isolation 3 How does ion exchange chromatography contribute to the purification of proteins from spinach leaves and what property of proteins does it exploit 4 Explain the role of NaCl in ion exchange chromatography and how it helps to elute proteins from the column 5 What analytical technique is used to determine protein concentration during ion exchange chromatography and why is this important in the process
8 Drawn below is part of the tertiary structure of a protein showing the positions of two amino acids aspartic acid and lysine Replacing lysine with another amino acid in the protein may change the shape and function of the protein Replacing lysine with which type s of amino acid s would lead to the least amount of change in the tertiary structure of this protein Interactions that determine the tertiary structure of proteins CH Hydrogen bond be chain and puppe donde www Pomp 04 1 Die bond
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Biotechnology: Principles and Processes
8 Drawn below is part of the tertiary structure of a protein showing the positions of two amino acids aspartic acid and lysine Replacing lysine with another amino acid in the protein may change the shape and function of the protein Replacing lysine with which type s of amino acid s would lead to the least amount of change in the tertiary structure of this protein Interactions that determine the tertiary structure of proteins CH Hydrogen bond be chain and puppe donde www Pomp 04 1 Die bond
Below is a polynucleotide is this DNA or RNA Why Is this a double stranded ds or a single stranded ss molecule Label the 5 and 3 end s of this molecule The convention is to write nucleic acid sequences in the 5 to 3 direction Thus wha nucleotide sequence of the polynucleotide shown using 1 letter abbreviation What type of bond maintains the structure of the primary structure
Biology
Biotechnology: Principles and Processes
Below is a polynucleotide is this DNA or RNA Why Is this a double stranded ds or a single stranded ss molecule Label the 5 and 3 end s of this molecule The convention is to write nucleic acid sequences in the 5 to 3 direction Thus wha nucleotide sequence of the polynucleotide shown using 1 letter abbreviation What type of bond maintains the structure of the primary structure
estion 5 molecules removes a unit of water from a system and joins two molecules together 2 points is when water is forced into a bond breaking it and forming two smaller Save Ansva
Biology
Biotechnology: Principles and Processes
estion 5 molecules removes a unit of water from a system and joins two molecules together 2 points is when water is forced into a bond breaking it and forming two smaller Save Ansva
Which molecule undergoes simple diffusion Na Glucose Amino acid Carbon dioxide Question 2 0 75 points Listen Saturated fats contain more double bond than unsaturated fats contain more hydrogen atoms than unsaturated fats contain more carbon atoms than unsaturated fats contain less hydrogen atoms than unsaturated fats
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Biotechnology: Principles and Processes
Which molecule undergoes simple diffusion Na Glucose Amino acid Carbon dioxide Question 2 0 75 points Listen Saturated fats contain more double bond than unsaturated fats contain more hydrogen atoms than unsaturated fats contain more carbon atoms than unsaturated fats contain less hydrogen atoms than unsaturated fats
Most antimicrobial compounds are going to be most effective when organisms are in O lag phase O exponential phase O stationary phase death phase
Biology
Biotechnology: Principles and Processes
Most antimicrobial compounds are going to be most effective when organisms are in O lag phase O exponential phase O stationary phase death phase
In which phase or phases of the growth curve is there very little change in total viable living cell numbers SELECT ALL THAT APPLY meaning multiple answers may be correct Lag Exponential Stationary
Biology
Biotechnology: Principles and Processes
In which phase or phases of the growth curve is there very little change in total viable living cell numbers SELECT ALL THAT APPLY meaning multiple answers may be correct Lag Exponential Stationary
The shortest lag period would most likely be observed in the transfer of a microbial culture O from a complex medium to a fresh complex medium O from a complex medium to fresh minimal medium O from a complex medium where the culture was growing at a high temperature to fresh complex media where the culture will be growing a lower temperature from a complex medium at a pH 7 0 to a fresh complex medium at a pH of 5 0
Biology
Biotechnology: Principles and Processes
The shortest lag period would most likely be observed in the transfer of a microbial culture O from a complex medium to a fresh complex medium O from a complex medium to fresh minimal medium O from a complex medium where the culture was growing at a high temperature to fresh complex media where the culture will be growing a lower temperature from a complex medium at a pH 7 0 to a fresh complex medium at a pH of 5 0
From the graph below the reaction that has the highest rate of product formed is the one catalyzed by Activation Energy Cat BHD Uncatalyzed Erayma A Erazyme B Reaction Progress Question 36 1 point 4 Listen AG AH TAS ZC H0 Enzyme A Enzyme B Uncatalyzed O both enzyme A and uncatalyzed reactions What can be said about an endothermic reaction with a negative entropy change O spontaneous at all temperatures Ospontaneous at high temperatures spontaneous in the reverse direction at all temperatures
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Biotechnology: Principles and Processes
From the graph below the reaction that has the highest rate of product formed is the one catalyzed by Activation Energy Cat BHD Uncatalyzed Erayma A Erazyme B Reaction Progress Question 36 1 point 4 Listen AG AH TAS ZC H0 Enzyme A Enzyme B Uncatalyzed O both enzyme A and uncatalyzed reactions What can be said about an endothermic reaction with a negative entropy change O spontaneous at all temperatures Ospontaneous at high temperatures spontaneous in the reverse direction at all temperatures
bind to the active site of an enzyme through noncovalent bonds are often toxic to cells have only a temporary effect on enzyme activity are not active against ribozymes Question 24 1 point Listen An enzyme is active in the stomach of an animal but quickly loses its activity when it leaves the stomach This example illustrates that enzymes are Odigested in the small intestine O consumed by the quantities of substrate in the small intestine inactivated by movement
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Biotechnology: Principles and Processes
bind to the active site of an enzyme through noncovalent bonds are often toxic to cells have only a temporary effect on enzyme activity are not active against ribozymes Question 24 1 point Listen An enzyme is active in the stomach of an animal but quickly loses its activity when it leaves the stomach This example illustrates that enzymes are Odigested in the small intestine O consumed by the quantities of substrate in the small intestine inactivated by movement
Page 97 5 4 Describe the role of enzymes in metabolic pathways A genetic mutation results in a change to the three dimensional shape of a photosynthetic enzyme s active site Which of the following is are likely the result s Correct Answer s This enzyme s shape is not affected by pH The associated metabolic reaction takes more energy The substrate molecules cannot attach to the enzyme The enzyme now must use an induced fit reaction There would be no effect on normal enzyme function Answer s
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Biotechnology: Principles and Processes
Page 97 5 4 Describe the role of enzymes in metabolic pathways A genetic mutation results in a change to the three dimensional shape of a photosynthetic enzyme s active site Which of the following is are likely the result s Correct Answer s This enzyme s shape is not affected by pH The associated metabolic reaction takes more energy The substrate molecules cannot attach to the enzyme The enzyme now must use an induced fit reaction There would be no effect on normal enzyme function Answer s
ePage s 93 94 Drag the molecules to their best matches below Drag each item on the left to its matching item on the right Note that every item may not have a match while some items may have more than one match ADP ATP contains two phosphate groups higher mass molecule lower mass molecule
Biology
Biotechnology: Principles and Processes
ePage s 93 94 Drag the molecules to their best matches below Drag each item on the left to its matching item on the right Note that every item may not have a match while some items may have more than one match ADP ATP contains two phosphate groups higher mass molecule lower mass molecule
What are the high energy products of this cycle FAD Click or tap the appropriate places in the image Pyruvate OOO FADH ADP P ATP CO NAD NADH
Biology
Biotechnology: Principles and Processes
What are the high energy products of this cycle FAD Click or tap the appropriate places in the image Pyruvate OOO FADH ADP P ATP CO NAD NADH
A useful technique for making millions of copies of a specific region of DNA is a Restriction enzymes Ob Gel electrophoresis Oc Spectrophotometry d Polymerase chain reaction
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Biotechnology: Principles and Processes
A useful technique for making millions of copies of a specific region of DNA is a Restriction enzymes Ob Gel electrophoresis Oc Spectrophotometry d Polymerase chain reaction
Before returning to the original lane after passing another vehicle you should Beep your horn See both headlights of the passed vehicle in your rear view mirror
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Biotechnology: Principles and Processes
Before returning to the original lane after passing another vehicle you should Beep your horn See both headlights of the passed vehicle in your rear view mirror
6 Using dotted lines now construct isodrosotherms at 5 F intervals on the simplified map in Figure 9 5 39 31 41 33 49 38 O O 34 22 38 28 O 41 30 e 42 36 48 38 50 O 38 O 52 50 40 38 48 44 45 60 40 55 61 57 66 59 45 43 63 61 66 60 48 45 O 57 54 61 57 39 39 70 59 69 61 66 53 39 39 43 41 46 43 54 45 66 55 60 72 64 O O 43
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Biotechnology: Principles and Processes
6 Using dotted lines now construct isodrosotherms at 5 F intervals on the simplified map in Figure 9 5 39 31 41 33 49 38 O O 34 22 38 28 O 41 30 e 42 36 48 38 50 O 38 O 52 50 40 38 48 44 45 60 40 55 61 57 66 59 45 43 63 61 66 60 48 45 O 57 54 61 57 39 39 70 59 69 61 66 53 39 39 43 41 46 43 54 45 66 55 60 72 64 O O 43
Which of the following is true of geometric isomers O they have different molecular formulas O they have variations in arrangement around a double bond O they have identical chemical properties O they are rare in nature
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Biotechnology: Principles and Processes
Which of the following is true of geometric isomers O they have different molecular formulas O they have variations in arrangement around a double bond O they have identical chemical properties O they are rare in nature
und 2 If having a capsule is an important advantage for certain pathogens then why wouldn t evolution favor all pathogens being encapsulated
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Biotechnology: Principles and Processes
und 2 If having a capsule is an important advantage for certain pathogens then why wouldn t evolution favor all pathogens being encapsulated
Select the feature s of an acidic dye select all that apply a Alkaline b Basic c Negatively charged d Includes India ink e Lacks a chromogen
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Biotechnology: Principles and Processes
Select the feature s of an acidic dye select all that apply a Alkaline b Basic c Negatively charged d Includes India ink e Lacks a chromogen
Which of the following are basic elements of the U S political culture 1 Economic equality II Individualism III Civic duty O II and III only O I only O III only I only
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Biotechnology: Principles and Processes
Which of the following are basic elements of the U S political culture 1 Economic equality II Individualism III Civic duty O II and III only O I only O III only I only
One reason why public policy differs from public opinion is public opinion tends to change slower than public policy that while public opinion is easy to gauge public policy is not The U S Constitution places O many checks on the influence of public opinion Public opinion polling tends to express the opinions of the political elite
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Biotechnology: Principles and Processes
One reason why public policy differs from public opinion is public opinion tends to change slower than public policy that while public opinion is easy to gauge public policy is not The U S Constitution places O many checks on the influence of public opinion Public opinion polling tends to express the opinions of the political elite
e 43 45 3 1 Compare and contrast the types of bonds used to form molecules Identify the location s on this image where covalent bonds can form Click or tap the appropriate place in the image Carbon atom
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Biotechnology: Principles and Processes
e 43 45 3 1 Compare and contrast the types of bonds used to form molecules Identify the location s on this image where covalent bonds can form Click or tap the appropriate place in the image Carbon atom
1 a Draw a recombinant Ti plasmid labeled cointegrate Ti plasmid on slide in Video 5 1 with all the components needed to use it to generate a transgenic plant T DNA borders selectable marker and transgene b Describe in a few words or one sentence the function or significance of each of these components T DNA borders Transgene
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Biotechnology: Principles and Processes
1 a Draw a recombinant Ti plasmid labeled cointegrate Ti plasmid on slide in Video 5 1 with all the components needed to use it to generate a transgenic plant T DNA borders selectable marker and transgene b Describe in a few words or one sentence the function or significance of each of these components T DNA borders Transgene
did the system of fixed Why did exchange rates eventually become obsolete contributing to O international tensions in some cases almost to war It was It did not accurately reflect a O constantly changing economic climate It allowed each nation to devalue its currency which led to worldwide inflation Too many nations were simply O following the lead of the United
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Biotechnology: Principles and Processes
did the system of fixed Why did exchange rates eventually become obsolete contributing to O international tensions in some cases almost to war It was It did not accurately reflect a O constantly changing economic climate It allowed each nation to devalue its currency which led to worldwide inflation Too many nations were simply O following the lead of the United
The O objectives nosepiece 58 arm stage is the flat surface on which microscope slides are placed for viewing
Biology
Biotechnology: Principles and Processes
The O objectives nosepiece 58 arm stage is the flat surface on which microscope slides are placed for viewing
What is the main structural component of the cell membrane and why is it important for the cell membrane to be selectively permeable
Biology
Biotechnology: Principles and Processes
What is the main structural component of the cell membrane and why is it important for the cell membrane to be selectively permeable
Which of the following years will have an off year election O 2012 O 2016 O 2019 O 2020
Biology
Biotechnology: Principles and Processes
Which of the following years will have an off year election O 2012 O 2016 O 2019 O 2020
and crack yo heels Dat s de good ole Cairo at las I jis knows it I say I ll take the canoe and go and see Jim It mightn t be you know Resource gutenberg org cache epub 76 pg76 tx Identify the technique used in the above conversation O Dialect O Soliloquy O Parallel episode
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Biotechnology: Principles and Processes
and crack yo heels Dat s de good ole Cairo at las I jis knows it I say I ll take the canoe and go and see Jim It mightn t be you know Resource gutenberg org cache epub 76 pg76 tx Identify the technique used in the above conversation O Dialect O Soliloquy O Parallel episode
Write the systematic name of each organic molecule HO HO structure OH OH OH OH name 1 3 propanediol butanol propane 1 2 3 triol
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Biotechnology: Principles and Processes
Write the systematic name of each organic molecule HO HO structure OH OH OH OH name 1 3 propanediol butanol propane 1 2 3 triol
Facts are stated in the per molecule In the third column of the table write the systematic IUPAC name of each alcohol Also in the drawing area below the table draw the chemical structure of 3 You can draw a condensed or skeletal structure whichever you prefer Part 1 of 2 alcohol 1 2 3 Part 2 of 2 some facts A common name for this alcohol is isopropyl alcohol This toxic alcohol unfortunately has a sweet taste that is attractive to animals and children It s often dyed an ugly color to discourage accidental ingestion Drinking as little as 10 ml of this alcohol can cause permanent blindness and as little as 50 ml can cause death Molecular structure of 3 H C OH CH U C X 8 systematic IUPA P name propan 2 ol methanol methanol
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Biotechnology: Principles and Processes
Facts are stated in the per molecule In the third column of the table write the systematic IUPAC name of each alcohol Also in the drawing area below the table draw the chemical structure of 3 You can draw a condensed or skeletal structure whichever you prefer Part 1 of 2 alcohol 1 2 3 Part 2 of 2 some facts A common name for this alcohol is isopropyl alcohol This toxic alcohol unfortunately has a sweet taste that is attractive to animals and children It s often dyed an ugly color to discourage accidental ingestion Drinking as little as 10 ml of this alcohol can cause permanent blindness and as little as 50 ml can cause death Molecular structure of 3 H C OH CH U C X 8 systematic IUPA P name propan 2 ol methanol methanol
add the alcohol starting material to the left hand side of the reaction and the necessary reagents above or below the arrow Pea be made in one step from an alcohol without losing starting material to unwanted extra products If so If the alkyl halide can t be made in one step or can t be made in good yield because of competing reactions or unwanted byproducts check the box under the drawing area D C C C Click and drag to start drawing a structure X
Biology
Biotechnology: Principles and Processes
add the alcohol starting material to the left hand side of the reaction and the necessary reagents above or below the arrow Pea be made in one step from an alcohol without losing starting material to unwanted extra products If so If the alkyl halide can t be made in one step or can t be made in good yield because of competing reactions or unwanted byproducts check the box under the drawing area D C C C Click and drag to start drawing a structure X
Draw the condensed structure of 1 5 pentanediol CH OH CH CH CH CH OH
Biology
Biotechnology: Principles and Processes
Draw the condensed structure of 1 5 pentanediol CH OH CH CH CH CH OH
1 3 Which of the following best describes how enzymes affect chemical reactions O They take the place of one of the reactants O They dissolve the products of reactions They change the reactants into ions O They speed up the rate of reactions
Biology
Biotechnology: Principles and Processes
1 3 Which of the following best describes how enzymes affect chemical reactions O They take the place of one of the reactants O They dissolve the products of reactions They change the reactants into ions O They speed up the rate of reactions
2 Choose a company and describe what resources the company uses to produce its commodity
Biology
Biotechnology: Principles and Processes
2 Choose a company and describe what resources the company uses to produce its commodity
000 OOO OOO 8 C world markets are impacted by changes in market price large firms may be more efficient in producing the most good or service for the least cost monopolies producing goods O and services for a market with fixed prices industry dominated by a few suppliers who exercise some control over price and quantity
Biology
Biotechnology: Principles and Processes
000 OOO OOO 8 C world markets are impacted by changes in market price large firms may be more efficient in producing the most good or service for the least cost monopolies producing goods O and services for a market with fixed prices industry dominated by a few suppliers who exercise some control over price and quantity
During redox reactions O the loss of electrons from one substance is called reduction O the reduction of a substance does not need to be coupled to the oxidation of another substance O electrons are lost from one substance and added to another substance O protons from one molecule replace the electrons lost from another molecule a substance that gains electrons is said to be oxidized
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Biotechnology: Principles and Processes
During redox reactions O the loss of electrons from one substance is called reduction O the reduction of a substance does not need to be coupled to the oxidation of another substance O electrons are lost from one substance and added to another substance O protons from one molecule replace the electrons lost from another molecule a substance that gains electrons is said to be oxidized
Which one of the following is false DNA fingerprinting typically compares only a few selected portions of a DNA molecule A DNA fingerprint is a specific pattern of electrophoresis bands O The markers most often used in DNA fingerprinting are coding sections of DNA DNA fingerprinting can be used to establish family relationships DNA fingerprints are now accepted as compelling evidence by legal experts and scientists
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Biotechnology: Principles and Processes
Which one of the following is false DNA fingerprinting typically compares only a few selected portions of a DNA molecule A DNA fingerprint is a specific pattern of electrophoresis bands O The markers most often used in DNA fingerprinting are coding sections of DNA DNA fingerprinting can be used to establish family relationships DNA fingerprints are now accepted as compelling evidence by legal experts and scientists
To teach the necessity of respect for the rights of persons and property as fundamental to every form of government to teach the duty of government to encourage and protect individual and group initiative and enterprise to foster the right to work earn save and acquire property and to preserve the ownership and lawful use of property when acquired Based on this statement and your own knowledge this mission statement belongs to O the John Birch Society O the American Liberty League O the Chamber of Commerce O the Sons of Liberty Complete Later Complete Que 1 5 9
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To teach the necessity of respect for the rights of persons and property as fundamental to every form of government to teach the duty of government to encourage and protect individual and group initiative and enterprise to foster the right to work earn save and acquire property and to preserve the ownership and lawful use of property when acquired Based on this statement and your own knowledge this mission statement belongs to O the John Birch Society O the American Liberty League O the Chamber of Commerce O the Sons of Liberty Complete Later Complete Que 1 5 9
Who was a Republican Party candidate for president in 1984 O Walter Mondale O Jimmy Carter O Ronald Reagan O Geraldine Ferraro
Biology
Biotechnology: Principles and Processes
Who was a Republican Party candidate for president in 1984 O Walter Mondale O Jimmy Carter O Ronald Reagan O Geraldine Ferraro
DNA Extraction of Your Samples for PCR 1 Prepare three samples Your food sample the GMO positive sample and the GMO negative sample Reducing the chance of contamination is critical Wipe all tools used micropestle and razor blade with alcohol wipes and do not switch tools between samples Crush or dice up a small amount of your sample with a micropestle or razor blade Add the crushed sample to a clean 1 5 mL Eppendorf tube to a level about halfway to the 0 1 mL mark Label the tube with the sample type and your group color Repeat the process for the GMO negative and GMO positive sample 2 Add 100 L of Edward s buffer to each tube containing the plant or food material Remember to change tips between samples to decrease the chance of contamination 3 Twist a clean micropestle against the inner surface of the 1 5 mL Eppendorf tube to forcefully grind the plant tissue or food product for 1 min 4 Add an additional 900 L of Edward s buffer to each tube containing the ground sample Grind briefly to remove tissue from the micropestle Vortex the tubes for 5 sec 5 6 Boil the samples for 5 min in a water bath using a circular floater Wrap tube openings with parafilm or use lid locks to keep lids from popping open during heating 7 Place the tubes in a balanced microcentrifuge and spin for 2 min at 12 000 rpms to pellet cell and food debris 8 Transfer 350 L of each supernatant to a fresh tube Maintain labels for each sample type and group color Be careful not to disturb the pelleted debris when transferring the supernatant Discard old tubes containing the precipitates 9 Add 400 L of isopropanol to each tube of supernatant 10 Mix by inverting the tubes several times and leave at room temperature for 3 min 11 Place the tubes in a balanced microcentrifuge and spin for 5 min at 14 000 rpms Align tubes in the rotor with the cap hinges pointing outward Nucleic acids will collect on the tube side under the hinge and pellet out of solution during centrifugation 12 Carefully pour off the supernatant from each tube and then completely remove the remaining liquid with a micropipette set at 100 uL
Biology
Biotechnology: Principles and Processes
DNA Extraction of Your Samples for PCR 1 Prepare three samples Your food sample the GMO positive sample and the GMO negative sample Reducing the chance of contamination is critical Wipe all tools used micropestle and razor blade with alcohol wipes and do not switch tools between samples Crush or dice up a small amount of your sample with a micropestle or razor blade Add the crushed sample to a clean 1 5 mL Eppendorf tube to a level about halfway to the 0 1 mL mark Label the tube with the sample type and your group color Repeat the process for the GMO negative and GMO positive sample 2 Add 100 L of Edward s buffer to each tube containing the plant or food material Remember to change tips between samples to decrease the chance of contamination 3 Twist a clean micropestle against the inner surface of the 1 5 mL Eppendorf tube to forcefully grind the plant tissue or food product for 1 min 4 Add an additional 900 L of Edward s buffer to each tube containing the ground sample Grind briefly to remove tissue from the micropestle Vortex the tubes for 5 sec 5 6 Boil the samples for 5 min in a water bath using a circular floater Wrap tube openings with parafilm or use lid locks to keep lids from popping open during heating 7 Place the tubes in a balanced microcentrifuge and spin for 2 min at 12 000 rpms to pellet cell and food debris 8 Transfer 350 L of each supernatant to a fresh tube Maintain labels for each sample type and group color Be careful not to disturb the pelleted debris when transferring the supernatant Discard old tubes containing the precipitates 9 Add 400 L of isopropanol to each tube of supernatant 10 Mix by inverting the tubes several times and leave at room temperature for 3 min 11 Place the tubes in a balanced microcentrifuge and spin for 5 min at 14 000 rpms Align tubes in the rotor with the cap hinges pointing outward Nucleic acids will collect on the tube side under the hinge and pellet out of solution during centrifugation 12 Carefully pour off the supernatant from each tube and then completely remove the remaining liquid with a micropipette set at 100 uL