Biotechnology & its Applications Questions and Answers

Which of the following would not be true of cDNA produced using human brain tissue as the starting material? A.It could be amplified by the polymerase chain reaction. B.It was produced from mRNA using reverse transcriptase. C.It could be used as a probe to detect genes expressed in the brain. D.It could be used to create a complete genomic library. E.It lacks the introns of the human nuclear genes.

Genomic imprinting is generally due to the addition of methyl (-CH3) groups to C nucleotides (as well as chemical changes to histone proteins) in order to silence a given gene. If the process of transmission of these features to the next generation, which affects all the progeny of the transmitting parent, depends on the sex of the parent who transmits the gene, which of the following must be true? A. Methylation of C is permanent in a gene. B.Genes required for early development stages must not be imprinted. C.Methylation of this kind must occur more in males than in females. D.The imprints are transmitted only to gamete-producing cells. E. Methylation must be reversible i ovarian and testicular cells.

The Federal Bureau of Investigation (FBI) maintains a database system including software to run it called by the acronym CODIS, for "Combined DNA Index System." (A) What is the main type of DNA data stored in this system and (B) how might this data be used to prove the guilt or innocence of a rape suspect?

Which of the following is true about NoSQL databases? Eventually the database will be consistent, but it's not guaranteed at any specific moment in time NoSQL works faster on big data because it uses data partitioning and distributed data storage All of these statements are true Users can access the database while other users are changing it, but they might have different views of the data

Which of the following statements is true when comparing moist heat methods of microbial control to dry heat control? Both moist heat and dry heat methods can achieve complete sterilization. Only moist heat (autoclaving) can sterilize objects, dry heat is limited to disinfection. Dry heat is more efficient than moist heat, requiring less time to sterilize objects. Boiling is a more successful method of sterilizing instruments than using a dry heat oven.

4. What best explains how PCR can be used to detect an infection? Specific temperatures are used to amplify DNA from an infectious agent if it was present in the patient. Specific restriction enzymes are used to cut the DNA from an infectious agent if it was present in the patient. That fragment can then be seen on a gel. Specific primers are used that would result in the amplification of DNA from an infectious agent if it was present in the patient. That amplification can be seen with the presence of a specific size fragment when the PCR product is ran on a gel.

Generalized transduction differs from specialized transduction in that generalized transduction can only transfer certain genes generalized transduction can take placebetween different species while specialized transduction cannot generalized transduction transfers random DNA fragments while specialized does not generalized transduction take place in gram-positive and gram-negative bacteria, specialized can only occur in gram-negative bacteria

Which of the following is NOT true of immunoprecipitation? includes a denaturing step uses antibody-coated beads separates particles using an electric field good for isolating specific proteins from a solution

Which of the following microscopes use UV light? O Darkfield microscope O Brightfield microscope O Compound light microscope O Fluorescence microscope Scanning electron microscope

In the disc diffusion susceptibility test, as the antimicrobic diffuses through the media and the concentration gradient is created, where is the highest level of the antimicrobic? A. The highest level of antimicrobic is found closest to the disc. B. The highest levels of antimicrobic is found at the point on the plate furthest from the disc.

Robbie and Saurab are pre-med and pre-pharmacy students, respectively. They complain to their biology professor that they should not have to study plants because plants have little relevance to their chosen professions. Which of the following statements are correct with regard to what physicians and pharmacists need to know about plants? A) Crop plants often produce more seeds than their wild relatives. B) Land plants produce poisons and medicines. C) Land plants often provide food for pollinators. D) Crop plants can often interbreed with their wild relatives.

Which of the following is/are safety issues and ethics of using DNA technology? a. Genetically modified crops must be safe for consumption b. Determining who has access to someone's genetic information c. Genetically modified crops must be released into the environment d. a and b e. a, b and c

Restriction enzymes create when they cut DNA. a. Sticky ends b. Blunt ends c. Methylated ends d. a and b e. a, b and c

The use of an antibiotic-resistance gene on a plasmid used in genetic engineering makes a. replica plating possible. b.selection of the desired clone possible. c. the recombinant cell dangerous. d. the recombinant cell unable to survive. e. All of the answers are correct.

PCR can be used to isolate a portion of a particular gene because a. the DNA primer is specific. b. DNA can be electrophoresed. c. all cells have DNA. d. all cells have RNA.

The process of understanding gene function via computer-assisted analysis is a. bioinformatics. b. proteomics. c. reverse genetics. d. forensic microbiology. e. metagenomics.

You want to determine whether a person has a certain mutant gene. The process involves using a primer and a heat-stable DNA polymerase. This process is a. translation. b. restriction mapping. c. transformation. d. PCR. e. site-directed mutagenesis.

The rise of methicillin-resistant Staphylococcus aureus (MRSA) can be considered to be an example of artificial selection because humans purposefully raise MRSA in large fermenters in an attempt to make the bacteria ever-more resistant. humans synthesize methicillin and create environments in which bacteria frequently come into contact with methicillin. Humans are becoming resistant to bacteria by taking methicillin. S. aureus is cultivated by humans to replenish the soil with nutrients.