Biotechnology & its Applications Questions and Answers

O job opportunities and the weather sanitation and transportation family relations and job opportunities infrastructure and farming Previous 00 00 00 00 Ques 1 5 9

Question 2 Points 3 What is NOT an example of a political machine Pyramidal organization O Political favors O Public bids Control of local governments

kb 12 3 2 0 85 0 65 1111111110 3 kb 12 kb 2 kb 1 2 3 4 EtBr stained after run DNA standard 0 85 kb linear plasmid B insert cut from vec supercoiled plasm DNA standard linear plasmid 5 6 7 8 EtBr in gel Use the data in the gel on the previous page FROM THE GEL ON THE LEFT to determine the standard curve and size of insert cut of vector shown by arrow and supercoiled plasmid Standard insert cut from vec cm Distance from origin supercoiled plasmi top direction of migration bottom 0 65 kb Insert Cut from vector Supercoile d Plasmid DNA size Plot the standard DNA on the below graph to give a standard curve Use the graph to determine the size of the plasmid DNA show how you determined the size of the plasmid DNA on the graph And report the size of the plasmid in the last row of the above table 10000 1000 BASE PAIRS 100 10 10 15 20 25 30 DNA STANDARD CURVE plate LB LB AMP LB AMP ARA Explanation 35 40 45 50 55 60 65 70 75 80 85 40 95 100 Distance Migrated 8 A In performing the transformation experiment with rpARA plasmid give two reasons why the following results might have happened 4 Experiment 1 Plasmid Growth 100 white colonies 100 white colonies Plasmid Growth No growth No growth

Long ears in rabbits is an autosomal dominant trait You cross a rabbit that is heterozygous for long ears with a rabbit that is homozygous for normal ears What is the probability that their first offspring will have long ears O 50 O 30 O 25 O 15 0

Question 6 Points 1 Why were schools mainly private in the 19th century O Public education was not mandatory Only rich people had access to it Funds were mainly directed to private schools O Political interests promoted private education only

October 29 1929 is known as O Black Tuesday The beginning of the Great Depression O The unofficial beginning to the Dow Jones Industrial Average Both Black Tuesday and the honin

4 Examine the following sequence Note that only one of the complementary strands is shown You do not have to write in the complementary strand I 5 atgcacgcag ttttacttgc ttcttcaaga gettatggag ccaagaaaaa gtcacttcac cctactggga aaacacaaaa gctccttaaa gtaccgaagg www

a If positive control is homozygous recessive would you be able to determine if restriction digestion was effective b Forward and reverse primers complementary would anneal rather than anneal to the DNA target c What are the major components of PCR Dilution questions serial dilutions percent solutions w v v v molarity a Prepare 1L DMEM 10 FBS 1 Glutamine 1 Pen strep b Prepare 200mL of 0 1 M CaCl from stock of 10M CaCl C

cells mL You dilute the broth by removing 1mL and adding 4mL of sterile medium to give you dilution tube 1 of bacterial cells You remove 1mL from this first dilution tube and add 9mL of sterile medium to give dilution tube 2 You remove 0 5mL from this second dilution tube and add 9 5mL of sterile medium to give dilution tube 3 Diagram what you performed in the dilutions State each dilution in terms of 1 relative to the previous tube dilution tube 1 Math dilution tube 2 Math dilution tube 3 Math State the overall dilution of tube 3 from the original broth What is the concentration of the bacterial cells in each of the three dilution tubes once the dilution is performed tube 1 Math

Analogical arguments are arguments based on comparison True False

Which of the following options best describes this sentence Life is like a box of chocolates An argumentative analogy Not an analogy An illustrative analogy

True False Question 8 2 points Listen Select ALL of the following statements that are true regarding sound localization It requires processing at the cortical level It uses time differences between sound reaching the two ears It is difficult to discriminate sound sources in the midline it from both ears

Visceral sensory neuron Integration center Receptor in viscera Response Motor neuron Visceral effector 1000 Stimulus PPearson

Examine the image How has this habitat changed after this dam was built What effects could this have for the animals living in and around the river Lower Sesan Il dam Lower Sesan Il dam

How are particulates harmful to the biosphere

The evolution of eukaryotic cells most likely involved evolution of an endomembrane system and subsequent evolution of mitochondria from a portion of the smooth endoplasmic reticulum endosymbiosis of an oxygen using bacterium in a larger bacterial host cell the endosymbiont evolved into chloroplasts endosymbiosis of an oxygen using bacterium in a larger bacterial host cell the endosymbiont evolved into mitochondria O endosymbiosis of a photosynthetic archaeal cell in a larger bacterial host cell to escape toxic oxygen the anaerobic archaea evolved into chloroplasts

18 Listen Which of the following happens first as a nephron processes blood Ofiltration O excretion O secretion Oreabsorption Oosmosis les are transferred Question 21 3 points

FRET was used to study the interaction between 2 molecules Fluorophores used were Cyan Fluorescent Protein CFP and Yellow Fluorescent Protein YFP CFP absorbs 430nm light and emits 500nm light YFP absorbs 500nm light and emits 580nm light The molecule onto which CFP binds to can act as O Fluorescent donor O Both as fluorescent donor and acceptor O Fluorescent acceptor It does not give off any fluorescence

Not all intercellular signals require transduction Which one of the following signals would be processed without transduction a signal that is weakly bound to a nucleotide a lipid soluble signal a signal that binds to a receptor in the cell membrane a signal that binds to the extracellular matrix

Which muscle is highlighted brachialis biceps brachii triceps brachii long head triceps brachii medial head Submit Request Answer LT M11 Deluxe Muscular Arm 6 part 3B Scientific P Pe

The most ancient branch point in animal phylogeny is that between having Oradial or bilateral symmetry true tissues or no tissues O Oa body cavity or no body cavity Oa well defined head or no head Odiploblastic or triploblastic embry

In a sprain the meniscus of the joint is stretched or torn requiring arthroscopic surgery to fix it True False

3 What is the MAIN purpose of a pre planning meeting O To introduce the team members to each other To determine the logistics of the actual person centered planning team meeting To discuss each of the team members who will be attending the actual person centered planning meeting To prepare chairs and tables in the office where the actual person centered planning meeting will take place 4 In contrast to traditional treatment planning person centered planning focuses on The person s social deficits The person s functional limitations The person s strengths and abilities The person s problems and inabilities

hen a person is prescribed an antibiotic for a bacterial illness but they stop taking the antibiotic before they finish it what population of bacteria remain in their be the ones most sensitive to the antibiotic the ones most resistant to the antibiotic a mixture of the ones most sensitive and resistant to the antibiotic there is no way to predict ones that will remain all of these

The best experimental design alters only one condition between the controls and the experimental condition includes a large sample size for each condition includes a control includes a large sample size and a control and alters only one condition between the controls and the experimental condition

1 A 25 5 liter balloon holding 3 5 moles of carbon dioxide leaks If we are able to determine that 1 9 moles of carbon dioxide escaped before the container could be sealed what is the new volume of the container 2 If Sample 1 contains 2 98 moles of hydrogen at 35 1 degrees C and 2 3 atm in a 32 8 L container How many moles of hydrogen are in a 45 3 liter

7 Rheumatoid Arthritis usually affects joints bilaterally i e both the right and left wrist whereas Osteoarthritis often affects joints on one side only i e only the right wrist in someone right handed For both diseases explain why

Ascomycota Zygomycota Chytrids Basidiomycota O yeasts Question 13 1 point Listen Fungi are classified as species that are often referred to as molds

Page 1 799 Listen Which of the following cellular structures is characteristic of OA microvilli OB cilia OC pseudopodia OD flagella

Restriction enzymes are biological that are used for various biotechnology techniques such as DNA fingerprinting A scissors C catalysts B needles D plasmids

Biotechnology involves the manipulation of organisms material for human purposes A specific alleles C operons B tRNA and mRNA D genetic

The attachment of methyl groups to DNA is a type of control in eukaryotic gene expression A post translational C pre translational B post transcriptional D pre transcriptional

A normal human karyotype shows chromosomes A 46 pairs of B 22 pairs of C 46 D 23

The reaction cascade in glycolysis cannot continue without unloading hydrogen from which molecule A NADH B NAD C ATP

The mechanism that permits immunoglobulins to be synthesized in either a membrane bound or secreted form is O allelic exclusion O codominant expression O class switching differential mRNA processing

11 The diagrams below represent some steps in a procedure used in biotechnology Bacterial DNA X 0 Y Foreign DNA Letters X and Y represent the 1 hormones that stimulate the replication of bacterial DNA 2 biochemical catalysts involved in the insertion of genes into other organisms 3 hormones that trigger rapid mutation of genetic information 4 gases needed to produce the energy required for gene manipulation

Which of the following is an incorrect statement GM plants may help with the problem of malnutrition in less developed countries GM plants may grow in a new variety of climates Genetically engineered plants may grow larger than the original organism Scientists are fully aware of the consequences of genetically modified organism

no heat is used and Stain doesn t enter the is more Precise than a direct Stain 2 Why were you instructed to discard the toothpick in disinfectant CRITICAL THINKING 1 What microscopic technique gives a field similar in appearance to that seen in the negative stain 2 Carbolfuchsin can be used as a direct stain and as a negative stain As a direct stain the pH is 3 Unlike nigrosin acidic Congo red does not contain suspended particles but it can give the appearance of a negative stain What is the basis for this stain CLINICAL APPLICATION Treponema denticola is easily missed in a direct stain of a tooth or gum scraping because the cells are distorted by the staining procedure Why can this bacterium be seen with negative staining ART CREDITS All ill hu Pracision Graphics unless otherwise noted

What kind of genetic information is most likely to be found on a plasmid Duplication of the entire genome Essential O Non essential Impossible to predict

client weighs 135 10 zero Blank 1 Blank 1 Add your answer Question 6 O 1 Point A nurse is preparing to administer furosemide 30 mg IV stat Available is furosemide 100 mg 10 mL How many mL should the nurse administer Reford answer as a whole number Do not use a trailing zero Blank 1

A medication order states administer acetaminophen 975 mg PO now Available is acetaminophen 325 mg tablet How many tablet s of Acetaminophen should the nurse administer Record answer as a whole number Do not use a trailing zero Blank 1 Blank 1 Add your answer Question 2 1 Point A medication order states administer amoxicillin oral suspension 300 mg PO stat Available is amoxicillin oral suspension 200 mg 5 mL How many mL should the nurse administer Record answer to the tenth or one decimal place Do not use a trailing zero Blank 1

Two of the major amino acids used for the synthesis of flavonoids are Phenylalanine and tyrosine Tryptophan and tyrosine Tryptophan and proline Tryptophan and histidine

How many tablets will be administered B 1 5 tablets 3 5 tablets A Question 6 B 2 tablets Order 1 5 mg Supply 2 mg per mL How many mL will be administered G 2 5 tablets 1 5 mL 1 75 mL 1 mL

Identify molecule whole molecule Peptide binding cleft 1 02 3 79997 8 8 8 8 8 8 8 8 U 8 8 8 LULE

D palactosidase pene 02 Ampicillin resialance pene amp Plasmid Rostriction ako Forelon DNA Rostriction ekos Flocombinont plasmid Bactorium Colonios with forelgr DNA In Figure 3 the bacteria transformed with the vector only without the gene of interest and plated on media containing ampicillin and X gal v O a form blue ampicillin resistant colonies O b form blue ampicillin sensitive colonies O c form white ampicillin resistant colonies O d form white ampicillin sensitive colonies Oe not grow

Listen A good example of a positive feedback mechanism would be blood calcium level regulation body temperature regulation regulating glucose levels in the blood enhancement of labor contractions

In a population of 25 individuals 11 are homozygous recessive What is q 011 11 25 11 25 squared

In the tables above you calculated the densities of a series of objects all made of the same material and calculated the average value standard deviation etc Based on these calculations answer the following questions 0 1pts 1 Is the coefficient of variation CV for the measured density 1 0 Is this a measurement of your technique s accuracy or of its precision Select the statement that best answers these questions A CV 1 0 measure of accuracy B CV 1 0 measure of precision C CV 1 0 measure of accuracy D CV 1 0 measure of precision 0 1pts 2 Is the percent error for the measured density 1 0 Is this a measurement of your technique s accuracy or of its precision Select the statement that best answers these questions A error 1 0 measure of accuracy B error 1 0 measure of precision C error 1 0 measure of accuracy D error 10 measure of precision Choose Choose

7 A notch cut into the connecting nut on the end of a welding hose indicates OA end of the hose should be connected to an oxygen tank OB end of the hose should be connected to an oxygen regulator OC hose is to be used only for acetylene OD nut has right hand threads

List 5 precautions that must be taken before beginning an experiment E T T B 1 U 0