The Living World Questions and Answers

We are doing an immunoblot and following color staining get the shown result What are some things you can modify in your procedure that would improve your result Select all that apply Increase staining time Increase the amount of washing in between incubations Decrease the protein in the blocking buffer Increase incubation time with Blotto Block with higher concentration of milk in Blotto

Which of the following statements would you expect to see as part of the discussion section O The Ras mitogen activated protein kinase MAPK signaling pathway is an evolutionarily conserved pathway involved in the control of many fundamental biological processes including cell proliferation apoptosis survival differentiation motility and metabolism GSH beads alone were unable to purify Mek1 Erk1 2 or glyceraldehyde 3 phosphate dehydrogenase GAPDH from the lysate OS2 cells used for the no treatment and insulin only treatment conditions were left in basal medium Schneider s medium with 10 fetal bovine serum during the preincubation OA similar observation of reduced phosphorylation of Erk2 despite activating phosphorylation of Mek1 has been made in cells expressing Mek1 mutants where the amino terminus of the protein a domain important for Erk2 interactions was deleted

The cell lysis solution was supplemented with phosphatase and protease inhibitors Protease inhibitors make sure that proteases do not destroy proteins within the sample What do phosphatase inhibitors do O Prevent phosphorylation of proteins in the sample O Prevent dephosphorylation of proteins in the sample O Prevent phosphate groups from being destroyed within the sample O Prevent the destruction of proteins within the sample

You probed for p53 protein in liver cancer samples from mice livers using a mouse polyclonal antibody against p53 How could this western be improved Select all that apply Reduce the concentration of secondary antibody Increase the concentration of secondary Reduce the concentration of primary antibody Increease the concentration of primary antibody Switch to a mouse monoclonal antibody

You are about to run an SDS PAGE gel but ran out of 1x running buffer You have a 20x stock solution You need 750 mL of 1x buffer to fill your chamber How much need stock solution and how much water will you O 37 5 ml 20x stock 750 ml water O 37 5 ml 20x stock 712 5 ml water 150 ml 20x stock 600 ml water O 25 ml 20x stock 725 ml water

You have conducted a standard curve and found the best fit trendline y 0 4524x 0 0183 Your blank corrected sample absorbance is 0 45 That sample was diluted 1 5 from the original concentration before it was run on the plate Your standard ranged from 0 2 2 0 mg ml What is the concentration of the undiluted sample 1 035 mg ml O 5 176 mg ml 0 185 mg ml 0 926 mg ml

31 From what source is the blood pictured below produced a A victim bleeding out b Splashes from moving blood c Passive drops from falling blood d Smeared blood from a struggle

O True O False QUESTION 30 way to describe a Cognitive Miser Studies on the effects of Mass Media Health Campaigns typically reveal statistically large effect sizes which equa large societal changes True False

communicating information quickly is almost always important because for members of the public it is common that the first CERC Principles addresses that crises are time sensitive and source of information often becomes the preferred source a Be Right Ob Be Credible O c Promote Action O d Express Empathy Oe Be First QUESTION 41 Health campaigns and interventions have the same general purpose True

follow O a 45 O b 25 O c 15 O d 55 O e 35 QUESTION 32 If you can get d Cultivation Theory of a population to shift or change the rest of the group will This theory states that mass media is not telling us exactly what or how to think but it does encourage people to connect certain dots on their own This theory states that mass media helps media consumers make associations in their own minds thereby shaping the considerations that people take into account when making judgments about various issues topics O a Agenda Setting Theory O b Social Comparison Theory O c Framing Theory

Which of the following is an example of an extinct species All choices are correct O Megalodon Tasmanian Tiger

Two different species can never interbreed and produce feritle offspring A True B False

Giraffes have long necks because the males use their long necks and reinforced bony skulls to fight with other males and they don t eat only leaves on A tall trees and in some areas they don t eat high leaves at all The male that wins gets to mate with the females and pass on his genes B they need long necks to reach high leaves on trees so they grew them some ancestral giraffes had long necks and some had short necks and the ones with the long necks were better able to survive because they could reach leaves on tall trees A combination of some giraffes had longer necks and were better able to survive because they could reach the tall trees and males fighting with other males for a mate contribute to the long necks

MAKING SENSE 5 Where is the Serengeti located in the continent of Africa 6 What predictions can we make about the climate temperature and precipitation of the Serengeti based on its location on the map I 7 Write two other observations you got from looking at the Serengeti Google Map CONCLUSION Use what we figured out today using the videos and the google map to answer the question below biodiversity in the Serengeti Cite at least 1 piece of evidence to justify 2

Read the following excerpt from Joy Harjo s poem Everybody Has a Heartache A Blues What feature of Postmodernism does this excerpt represent This silence in the noise of the terminal is a mountain of bison skulls Nobody knows nobody sees Unless the indigenous are dancing powwow all decked out in flash and beauty We just don t exist We ve been dispersed to an outlaw cowboy tale What were they thinking with all those guns and those handcuffs In a size for babies They just don t choose to remember We re here Truth is absolute and can be discovered through observation Utopian setting used to comment on contemporary life Blending of traditional and contemporary elements Decreasing contact with the actual physical world

4 What ideas do you have to explain why the population of buffalo increased so rapidly I INVESTIGATION PROCEDURE View the Serengeti National Park on Google Maps and the camera trap data below to gather more information about the location of the Serengeti and the animals that live there Then use these data sources along with your observations and takeaways from the video to answer the Making Sense Questions about the Serengeti MAKING SENSE 5 Where is the Serengeti located in the continent of Africa 6 What predictions can we make about the climate temperature and precipitation of the Serengeti the man

Write two other observations you got from looking at the Serengeti Google Map I CONCLUSION Use what we figured out today using the videos and the google map to answer the question below What can we conclude about the biodiversity in the Serengeti Cite at least 1 piece of evidence to justify your conclusions Example using Yellowstone According to the video clip after wolves were reintroduced many species of animals returned such as eagles beavers bears birds rabbits foxes and mice Now there is a high level of Biodiversity in Yellowstone National Park

BACKGROUND KNOWLEDGE 1 What do you know about the wildlife in Africa What types of animals are there I 2 What does the landscape environment look like African Buffalo Population 1960 1975 80000 Population Size 60000 40000

Someone says This grass is a primary consumer because it creates its own energy What would you say Your answer Someone says This deer is the first trophic level because it is a primary consumer What would you say Your answer Someone says I am an autotroph because I eat food What would you say Your answer om this lesson

Which organisms are secondary consumers Grasses Aspen Cottonwood Willow Mice Beavers Elk Deer Rough legged hawk Coyote Which organisms are heterotrophs Grasses Aspen Cottonwood Willow Mice Beavers Elk Deer Rough legged hawk Coyote Which organisms are autotrophs Grasses Aspen Cottonwood Willow Mice Beavers Elk Deer Rough legged hawk Coyote

Which organisms are the second trophic level Grasses Aspen Cottonwood Willow Mice Beavers Elk Deer Rough legged hawk Coyote Which organisms are the third trophic level Grasses Aspen Cottonwood Willow Mice Beavers Elk Deer Rough legged hawk Coyote What trophic level are the wolves O1st trophic level O2nd trophic level O 3rd trophic level 4th trophic level

Yellowstone National Park Trophic Cascade Trophic cascade Rough legged hawk O Mice Grasses Coyote Beavers Aspen Wolf Elk e Cottonwood Grasses Aspen Cottonwood Willow Mice Beavers Elk Deer Rough legged hawk Coyote Which organisms are the first trophic level Mule deer Willow Black arrows indicate an Increase in the consumption rate since the reintroduction of wolves Gray arrows indicate a decrease in the consumption rate since the reintroduction of wolves 1 point

What happened when wolves were absent for 70 years Wolves killed some of the deer Deer population increased Increase in birds beavers rabbits mice hawks foxes ravens bald eagles bears Vegetation was grazed away by deer Forests grew 1 point Riverbanks were stabilized by forests rivers changed course Deer started avoiding parts of the park What happened when the wolves were reintroduced Forests grew 1 point Wolves killed some of the deer Deer population increased Increase in birds beavers rabbits mice hawks foxes ravens bald eagles bears Vegetation was grazed away by deer Riverbanks were stabilized by forests rivers changed course Deer started avoiding parts of the park

Take a guess How do wolves change rivers

Throughout this class you have been educated on a sports games by taking notes doing homework tests research and performing activities Now it is your time to show off what you have learned Write 5 sentences on what you have learned from this class that pertains to the class Use the writing rubric on the wall in the room as your guide 5 points

4 Name and find the molar mass of the followings I CuSO4 5H O J NaHCO3 K Pb NO3 2

The House of Representatives Leader Speaker of the House Powers based on population bring impeachment charges President Pro Tempore when VP is gone The Senate Leader hold impeachment trials Powers approve treaties negotiated by President How the number of representatives is determined How the number of representatives is determined

Eyelash length is an inherited trait In the human population there is an eyelash length gene There are two possible variants of this gene an allele for long eyelashes 1cm and an allele for short eyelashes 1 cm or less The allele for long eyelashes is dominant L and the allele for short eyelashes is recessive I An individual who is heterozygous for eyelash length would have which of the following genotypes long eyelashes L1 11

What will happen if red blood cells are transferred to seawater which has a very high salt concentration Water will leave the cells causing them to shrivel and collapse Nothing will happen The blood cells will take up water swell and eventually burst

A defective ribosome would have the MOST detrimental effect on what processes O duplication O replication translation transcription

S 15 nce Figure What results from the chemical reaction illustrated in Figure O formation of an ionic bond Oformation of a hydrogen bond O formation of a covalent bond

Question 4 Blue flower color B allele is dominant to white b allele If a population of plants contains white flowers bb at a frequency of 0 04 what is the frequency of the blue flower B 0 2 or 20 O 0 8 or 80 0 32 or 32 0 64 or 64

In a particular plant leaf color is controlled by gene locus D Plants with at least one allele D have dark green leaves and plants with the homozygous recessive dd genotype have light green leaves A heterozygous genotype Dd is crossed with hetetrozygous Dd genotype and the predicted outcome is diagrammed in the Punnett square shown in Figure where 1 2 3 and 4 represent the genotypes corresponding to each box within the square Which of the boxes marked 1 4 correspond to plants with dark leaves 1 and 2 O2 and 3 O 4 only D D 1 2 d 3 1 2 and 3 d 2 4

18 The dragon on this sixteenth century bowl from the Chinese Ming Dynasty is best described as OA a cultural symbol OB an expression of personal identity OC intentionally frightening D purely decorative

22 What must Wade complete in order to pas stage two Is he worried about this challenge Why or why not

7 Describe Wade s initial thoughts reactions when he meets Aech in person 8 Morrow s Mansion and the surrounding area looks a lot like

3 What song holds the secret to entering the third gate 4 What request does Wade email to every single Oasis user

The underlined passage in The Invalid s Story is an example of Sec 31 I found out then that I had spent that awful night with a harmless box of rifles and a lot of innocent cheese but the news was too late to save me A Humor the situation of them losing their health is humorous B Verbal irony the narrator says they lost their health and they did lose their health C Situational irony in taking care of the deceased the characters lose their own health

What would you observe in a cell in which Primase activity was reduced to 50 of its normal levels ODNA replication would cease It would take twice as long to replicate all of the DNA O Replication of the leading strand would be almost normal but the lagging strand would take much longer to synthesize ODNA replication would proceed at a normal rate and the overall time for one round of replication would not be significantly changed

Shown below is a hypothetical replication bubble In what direction does the new strand that uses the bottom cyan DNA as its template grow in 3 00 From right to left From left to right 3 5

What would you observe in a cell in which DNA helicase was inactivated ODNA replication would fail due to the inability to separate the two strands of DNA DNA replication would occur on the leading strand but not the lagging strand DNA replication would begin but would eventually stall due to the increase in twisting beyond the replication fork DNA replication would fail due to the absence of a primer for DNA polymerization

If DNA replicated dispersively which of these most closely approximates what you would see after two generations if you were to perfc the Meselson Stahl experiment one band shown below two bands shown below 3 bands shown below

6 The game begins with a coin toss 7 If you are taking a right handed lay up what foot should you use to push off of the ground What is the saying used to help teach a basketball shot correctly han you are ch

Which toxin causes the epithelial cells lining the large intestine to secrete large amounts of water and electrolytes leading to watery diarrhea O Tetanus toxin Botulinum toxin O Cholera toxin Diphtheria toxin

3 9 10 T F The shot clock in box lacrosse is T F For most penalties the offending player is sent to the penalty box

Baseball Softball 1 Baseball and softball are considered 2 What is baseball and softball scored with 3 How many strikes does it take to receive an out receive a walk games

4 How many balls does it take to 5 How many innings do MLB players play 6 When the batter is hit by a pitch he receives a 7 How many players are on defense at one time in baseball our arms

Where have you experienced successes and a sense of accomplishment with school or with family relationships work etc This is a place for you to reflect on the hopeful aspects of life where things may be going smooth

The ball may be stopped with your hand but cannot be T F Ties will not stand T F Goalies are required to wear a facemask

T F Goalies are required to wear a facem 8 9 The penalty for intentional or unintentional hitting with a stick is 10 The penalty for using an elbow to push a player out of position is