Biotechnology: Principles and Processes Questions and Answers

Most antimicrobial compounds are going to be most effective when organisms are in O lag phase O exponential phase O stationary phase death phase

In which phase or phases of the growth curve is there very little change in total viable living cell numbers SELECT ALL THAT APPLY meaning multiple answers may be correct Lag Exponential Stationary

The shortest lag period would most likely be observed in the transfer of a microbial culture O from a complex medium to a fresh complex medium O from a complex medium to fresh minimal medium O from a complex medium where the culture was growing at a high temperature to fresh complex media where the culture will be growing a lower temperature from a complex medium at a pH 7 0 to a fresh complex medium at a pH of 5 0

From the graph below the reaction that has the highest rate of product formed is the one catalyzed by Activation Energy Cat BHD Uncatalyzed Erayma A Erazyme B Reaction Progress Question 36 1 point 4 Listen AG AH TAS ZC H0 Enzyme A Enzyme B Uncatalyzed O both enzyme A and uncatalyzed reactions What can be said about an endothermic reaction with a negative entropy change O spontaneous at all temperatures Ospontaneous at high temperatures spontaneous in the reverse direction at all temperatures

bind to the active site of an enzyme through noncovalent bonds are often toxic to cells have only a temporary effect on enzyme activity are not active against ribozymes Question 24 1 point Listen An enzyme is active in the stomach of an animal but quickly loses its activity when it leaves the stomach This example illustrates that enzymes are Odigested in the small intestine O consumed by the quantities of substrate in the small intestine inactivated by movement

ePage s 93 94 Drag the molecules to their best matches below Drag each item on the left to its matching item on the right Note that every item may not have a match while some items may have more than one match ADP ATP contains two phosphate groups higher mass molecule lower mass molecule

What are the high energy products of this cycle FAD Click or tap the appropriate places in the image Pyruvate OOO FADH ADP P ATP CO NAD NADH

A useful technique for making millions of copies of a specific region of DNA is a Restriction enzymes Ob Gel electrophoresis Oc Spectrophotometry d Polymerase chain reaction

Before returning to the original lane after passing another vehicle you should Beep your horn See both headlights of the passed vehicle in your rear view mirror

6 Using dotted lines now construct isodrosotherms at 5 F intervals on the simplified map in Figure 9 5 39 31 41 33 49 38 O O 34 22 38 28 O 41 30 e 42 36 48 38 50 O 38 O 52 50 40 38 48 44 45 60 40 55 61 57 66 59 45 43 63 61 66 60 48 45 O 57 54 61 57 39 39 70 59 69 61 66 53 39 39 43 41 46 43 54 45 66 55 60 72 64 O O 43

Which of the following is true of geometric isomers O they have different molecular formulas O they have variations in arrangement around a double bond O they have identical chemical properties O they are rare in nature

und 2 If having a capsule is an important advantage for certain pathogens then why wouldn t evolution favor all pathogens being encapsulated

Select the feature s of an acidic dye select all that apply a Alkaline b Basic c Negatively charged d Includes India ink e Lacks a chromogen

Which of the following are basic elements of the U S political culture 1 Economic equality II Individualism III Civic duty O II and III only O I only O III only I only

One reason why public policy differs from public opinion is public opinion tends to change slower than public policy that while public opinion is easy to gauge public policy is not The U S Constitution places O many checks on the influence of public opinion Public opinion polling tends to express the opinions of the political elite

e 43 45 3 1 Compare and contrast the types of bonds used to form molecules Identify the location s on this image where covalent bonds can form Click or tap the appropriate place in the image Carbon atom

1 a Draw a recombinant Ti plasmid labeled cointegrate Ti plasmid on slide in Video 5 1 with all the components needed to use it to generate a transgenic plant T DNA borders selectable marker and transgene b Describe in a few words or one sentence the function or significance of each of these components T DNA borders Transgene

did the system of fixed Why did exchange rates eventually become obsolete contributing to O international tensions in some cases almost to war It was It did not accurately reflect a O constantly changing economic climate It allowed each nation to devalue its currency which led to worldwide inflation Too many nations were simply O following the lead of the United

The O objectives nosepiece 58 arm stage is the flat surface on which microscope slides are placed for viewing

What is the main structural component of the cell membrane and why is it important for the cell membrane to be selectively permeable

and crack yo heels Dat s de good ole Cairo at las I jis knows it I say I ll take the canoe and go and see Jim It mightn t be you know Resource gutenberg org cache epub 76 pg76 tx Identify the technique used in the above conversation O Dialect O Soliloquy O Parallel episode

Write the systematic name of each organic molecule HO HO structure OH OH OH OH name 1 3 propanediol butanol propane 1 2 3 triol

Facts are stated in the per molecule In the third column of the table write the systematic IUPAC name of each alcohol Also in the drawing area below the table draw the chemical structure of 3 You can draw a condensed or skeletal structure whichever you prefer Part 1 of 2 alcohol 1 2 3 Part 2 of 2 some facts A common name for this alcohol is isopropyl alcohol This toxic alcohol unfortunately has a sweet taste that is attractive to animals and children It s often dyed an ugly color to discourage accidental ingestion Drinking as little as 10 ml of this alcohol can cause permanent blindness and as little as 50 ml can cause death Molecular structure of 3 H C OH CH U C X 8 systematic IUPA P name propan 2 ol methanol methanol

add the alcohol starting material to the left hand side of the reaction and the necessary reagents above or below the arrow Pea be made in one step from an alcohol without losing starting material to unwanted extra products If so If the alkyl halide can t be made in one step or can t be made in good yield because of competing reactions or unwanted byproducts check the box under the drawing area D C C C Click and drag to start drawing a structure X

Draw the condensed structure of 1 5 pentanediol CH OH CH CH CH CH OH

1 3 Which of the following best describes how enzymes affect chemical reactions O They take the place of one of the reactants O They dissolve the products of reactions They change the reactants into ions O They speed up the rate of reactions

2 Choose a company and describe what resources the company uses to produce its commodity

000 OOO OOO 8 C world markets are impacted by changes in market price large firms may be more efficient in producing the most good or service for the least cost monopolies producing goods O and services for a market with fixed prices industry dominated by a few suppliers who exercise some control over price and quantity

During redox reactions O the loss of electrons from one substance is called reduction O the reduction of a substance does not need to be coupled to the oxidation of another substance O electrons are lost from one substance and added to another substance O protons from one molecule replace the electrons lost from another molecule a substance that gains electrons is said to be oxidized

Which one of the following is false DNA fingerprinting typically compares only a few selected portions of a DNA molecule A DNA fingerprint is a specific pattern of electrophoresis bands O The markers most often used in DNA fingerprinting are coding sections of DNA DNA fingerprinting can be used to establish family relationships DNA fingerprints are now accepted as compelling evidence by legal experts and scientists

To teach the necessity of respect for the rights of persons and property as fundamental to every form of government to teach the duty of government to encourage and protect individual and group initiative and enterprise to foster the right to work earn save and acquire property and to preserve the ownership and lawful use of property when acquired Based on this statement and your own knowledge this mission statement belongs to O the John Birch Society O the American Liberty League O the Chamber of Commerce O the Sons of Liberty Complete Later Complete Que 1 5 9

Who was a Republican Party candidate for president in 1984 O Walter Mondale O Jimmy Carter O Ronald Reagan O Geraldine Ferraro

DNA Extraction of Your Samples for PCR 1 Prepare three samples Your food sample the GMO positive sample and the GMO negative sample Reducing the chance of contamination is critical Wipe all tools used micropestle and razor blade with alcohol wipes and do not switch tools between samples Crush or dice up a small amount of your sample with a micropestle or razor blade Add the crushed sample to a clean 1 5 mL Eppendorf tube to a level about halfway to the 0 1 mL mark Label the tube with the sample type and your group color Repeat the process for the GMO negative and GMO positive sample 2 Add 100 L of Edward s buffer to each tube containing the plant or food material Remember to change tips between samples to decrease the chance of contamination 3 Twist a clean micropestle against the inner surface of the 1 5 mL Eppendorf tube to forcefully grind the plant tissue or food product for 1 min 4 Add an additional 900 L of Edward s buffer to each tube containing the ground sample Grind briefly to remove tissue from the micropestle Vortex the tubes for 5 sec 5 6 Boil the samples for 5 min in a water bath using a circular floater Wrap tube openings with parafilm or use lid locks to keep lids from popping open during heating 7 Place the tubes in a balanced microcentrifuge and spin for 2 min at 12 000 rpms to pellet cell and food debris 8 Transfer 350 L of each supernatant to a fresh tube Maintain labels for each sample type and group color Be careful not to disturb the pelleted debris when transferring the supernatant Discard old tubes containing the precipitates 9 Add 400 L of isopropanol to each tube of supernatant 10 Mix by inverting the tubes several times and leave at room temperature for 3 min 11 Place the tubes in a balanced microcentrifuge and spin for 5 min at 14 000 rpms Align tubes in the rotor with the cap hinges pointing outward Nucleic acids will collect on the tube side under the hinge and pellet out of solution during centrifugation 12 Carefully pour off the supernatant from each tube and then completely remove the remaining liquid with a micropipette set at 100 uL

The government agency that regulates and licenses television and other communication industries is I Federal Communications Commission II Mass Media Commission III National Institute of Standards and Technology OI only O II only O III only O I and II only

Which of the following is not correct concerning President Truman 1 His second term was more successful than his first II Truman failed at creating irrigation projects III Truman was successful in creating over a million low income housing units OI only Oll only O Ill only OI and II only

Who used the concept of Surrealism in his work Franz Kafka a Czech writer O Marcel Proust a French novelist O Sigmund Freud the founder of modern psychology Oswald Spengler a German historian and philosopher

Civil Rights Act of 1964 National Organization for women Which of the following choices completes the chart above O Equal Employment Opportunity Commission O Declaration of Indian Purpose O National Farm Workers Association O National Council of Indian Opportunity

In 1954 Jim Crow Laws were challenged in which state O Tennessee O Louisiana O Kansas O Alabama

What is the prime aim of the G I Bill O To provide veterans with money for education housing and job training O To kill the capitalistic ideology O To improve the transportation O To concentrate the wealth within a small group

Choose the correct answer pioneered the new form of architecture in the postwar years He created a new style known as O Louis Sullivan functionalism O Salvador Dali surrealism O Georges Braque cubism Pier Mondrian abstract art form Complete Later Complete 1 5 9 13 17 21 25

A 1965 voter registration drive in Selma Alabama led by Dr Martin Luther King turned violent prompting the passage of O the Civil Rights Act of 1964 O the Civil Rights Act of 1968 O the 15th Amendment O the Voting Rights Act of 1965 Res Res this Que 5

Millions of East Germans flee to West Berlin Khrushchev threatens to close all roads Kennedy refuses to relent Construction of the Berlin Wall O Beginning of the Berlin Blockade O Beginning of the Berlin Airlift Question 1 S Rese 9

Your roommate is very messy and she or he never cleans the bathroom In disgust you decide you d like to show your roommate how dirty th bathroom is by estimating the number of bacterial species on the shower curtain What is the best method for answering this question By scraping the curtain and trying to grow the bacteria in cultures By applying the gram stain By scraping the curtain and counting the number of shapes under the microscope By directly sequencing DNA that you have scraped off the curtain By licking the shower curtain and cooing if you got cigle

True or False Epidemiologists test hypotheses by making comparisons usually within the context of a formal epidemiological study O True False

Question B Points 1 Identify the middle class reformer who opposed U S involvement in the war O Woodrow Wilson O jane Addams William Jennings Bryan O Fredj Hoertz

If you set up 1 3 serial dilutions of 1 00 M NaCl what is the concentration in Molar of the 3rd dilution tube Remember that the stock solution doesn t count as a dilution tube

5 4 points You have 5 x107 cells mL in a volume of 2 mLs a How many cell culture flasks can you seed at a density of 1 x 105 cells mL if each flasks will have a final volume of 5mL Answer Show calculation b How many mL of the resuspended cells will you seed into each flask Answer

6 2 points A procedure calls for 10ng of stock solution is 1 g DNA L How much stock do you need if your reaction mixture will be a total of 200 Ls Give answer in Ls Answer Show calculation 100 th picture for the

If an antigen that produces immunity on a pathogen is a carbohydrate would it be possible to create and use a DNA vaccine to induce long term immunity against that antigen For the toolbar press ALT F10 PC or ALT FN F10 Mac 11 T

dilation of the pupils salivation elimination of urine increased peristalsis of the digestive viscera Question 45 2 points 4 Listen a result of parasympathetic stimulation Preparing the body for the fight or flight response is the role of the parasympathetic division somatic nervous system cerebrum sympathetic division